Summya Rashid
Jamia Hamdard, India
Title: Chrysin attenuates 5- fluorouracil induced renal toxicity: Possible role of oxidative stress and apoptosis
Biography
Biography: Summya Rashid
Abstract
5-Fluorouracil (5-FU) is an effective anti-cancer drug commonly used for the treatment of various malignancies. It has varied undesirable effects such as hepatotoxicity, nephrotoxicity and cardio toxicity which limit its broad and widespread clinical practice. It is found to result in remarkable organ toxicities along with increased oxidative stress and apoptosis. Chrysin (CH) is a natural flavonoid having anti-oxidative, anti inflammatory and anti-cancerous activities. It is found in many plant extracts, blue passion flower, honey and propolis. The present study was designed to investigate the efficacy of CH against 5-FU induced renal toxicity in Wistar rats by biochemical, histopathological and immunohistochemical approaches. Rats were subjected to prophylactic oral treatment of CH for 21 days at lower dose of 50 mg/kg b.wt. and a higher dose of 100 mg/kg b.wt. against renal toxicity induced by single intra peritoneal injection of 5-FU (150 mg/kg bwt). The probable mechanism of 5-FU induced renal toxicity is the generation of free radicals resulting in oxidative stress; activation of apoptotic pathway by upregulation of p53, bax, caspase-3 and down regulating Bcl-2. On the contrary, prophylactic treatment of CH decreased renal serum toxicity markers, increased renal anti-oxidant armory as well as regulated apoptosis in kidneys of wistar rats. Histopathological alterations further confirmed the biochemical and immunohistochemical data. Hence, results of the present finding suggest that CH may be a positive modulator in alleviating 5-FU induced renal toxicity and may be used in combinational therapy before further pre clinical and clinical testing.